The truck hit the Child Care Network located at 1405 Friendly Avenue around 10:30 a.m.
Officials say the city-owned sanitation truck was traveling southbound on Hillcrest Road when it collided with a Honda Civic traveling eastbound on Friendly Avenue.
The truck continued through the parking lot and crashed into the side of the daycare, and through the exterior brick wall.
The truck crashed into the daycare kitchen.
The driver of the truck and a passenger were transported for minor injuries. The driver of the Honda was not injured.
Officials said 145 children were in the daycare at the time. None of the children were injured. The children are infants up to 6-years-old.
The incident closed all lanes of Friendly Avenue from Westover to Market Street for several hours.
Parents can pick up their children at West Market Street and Hillcrest Drive.
No charges have been filed.